pGEX2TK E7 DLYC
(Plasmid
#13672)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13672 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGEX2TK
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Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 4900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHPV 16 E7 del DLYC
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Alt nameHPV 16 E7
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SpeciesH. sapiens (human)
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Insert Size (bp)300
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Mutationdel aa 21-24
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GenBank IDAY089955
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Entrez GeneE7 (a.k.a. HpV16gp2)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer Amersham pGEX 5'
- 3′ sequencing primer Amersham pGEX 3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX2TK E7 DLYC was a gift from Karl Munger (Addgene plasmid # 13672 ; http://n2t.net/addgene:13672 ; RRID:Addgene_13672) -
For your References section:
The human papillomavirus E7 oncoprotein can uncouple cellular differentiation and proliferation in human keratinocytes by abrogating p21Cip1-mediated inhibition of cdk2. Jones DL, Alani RM, Munger K. Genes Dev. 1997 Aug 15. 11(16):2101-11. 10.1101/gad.11.16.2101 PubMed 9284049