pAAV-syn-F14F15S-splitTVA-EGFP-tTA
(Plasmid
#136917)
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PurposeThis vector has been DISCONTINUED. The updated version is Plasmid 183352: pAAV-syn-F14F15S-splitTVA-EGFP-tTAv2, linked in the comments below.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 136917 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAAV
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTVA, EGFP, tet transactivator
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SpeciesSynthetic
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Insert Size (bp)3648
- Promoter synapsin
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
THIS VECTOR IS DISCONTINUED. An updated version can be found here: https://www.addgene.org/183352/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-syn-F14F15S-splitTVA-EGFP-tTA was a gift from Ian Wickersham (Addgene plasmid # 136917)