Addgene - ACS2 Plasmid Data

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13747: ACS2
13746: ACS1
13673: pQLinkGD
13671: pQLinkG2
13670: pQLinkN

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Plasmid 13747: ACS2

Gene/insert name:
Acetyl CoA Sythetase 2
Alt name:
AceCS2
Insert size:
2046
Species:
M. musculus (mouse)
GenBank ID:
NM_080575
Entrez Gene:
Acss1 (Acas2, Acas2l, AceCS2, AI788978, 1110032O15Rik)
Fusion protein or tag:
His
Terminal:
N terminal on backbone
Mutation:
Full-length ACS2
Vector backbone:
pQE80
(Search Vector Database)
Backbone manufacturer:
Qiagen
Vector type:
Bacterial Expression
Backbone size w/o insert:
4700
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
SalI
Site destroyed during cloning:
No
5' sequencing primer:
pQE80 sequencing vector List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (1)
Principal Investigator:
John Denu
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Sirtuins deacetylate and activate mammalian acetyl-CoA synthetases. Hallows et al (Proc Natl Acad Sci U S A. 2006 Jul 5. 103(27):10230-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13747" in your Materials and Methods section.