Addgene - pCDNA3 Flag Jnk1b2 Plasmid Data

Plasmid 13752: pCDNA3 Flag Jnk1b2

Gene/insert name:
Jnk1b2
Alt name:
MAPK8 variant 4
Insert size:
1400
Species:
H. sapiens (human)
GenBank ID:
NM_139047
Entrez Gene:
MAPK8 (JNK, JNK-46, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1, SAPK1c)
Fusion protein or tag:
Flag
Terminal:
N terminal on insert
Vector backbone:
pCDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5446
Cloning site 5':
Hind III
Site destroyed during cloning:
No
Cloning site 3':
Xba I
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
Sp6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Roger Davis
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Selective interaction of JNK protein kinase isoforms with transcription factors. Gupta et al (EMBO J. 1996 Jun 3. 15(11):2760-70. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13752" in your Materials and Methods section.