pPGKPhiC31obpA
(Plasmid #13795)

• Purpose: Expression of mouse codon-optimized PhiC31 recombinase with bovine growth hormone polyadenylation (bpA) sequence.
• Depositing Lab: Philippe Soriano
• Publication: Raymond et al PLoS ONE. 2007 . 2():e162.

Backbone

• Vector backbone: na
• Backbone size w/o insert (bp): 3800
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PhiC31o
• Alt name: PhiC31
• Insert Size (bp): 1900

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: See map (not destroyed)
• 3′ cloning site: See map (not destroyed)
• 5′ sequencing primer: mPGK-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PhiC31o is a mouse codon-optimized PhiC31.