Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pLenti-Tet
(Plasmid #138086)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 138086 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Custom
  • Backbone size (bp) 8684
  • Vector type
    Mammalian Expression, Lentiviral
  • Promoter minimal CMV promoter with heptamerized tet‐operator
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid can be used in conjunction with pTet-CTRL (Addgene #138085) that expresses the Tet-transactivator proteins for controlled expression of the ORF for the gene of interest cloned into this plasmid. Following Lentiviral transduction into mammalian cells expression can be induced with Tetracycline or Doxycyline.
To clone the gene of interest into this plasmid, digest with BamHI and then perform Gibson assembly, adding your gene of interest including any tags.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-Tet was a gift from Eva Frickel (Addgene plasmid # 138086 ; http://n2t.net/addgene:138086 ; RRID:Addgene_138086)
  • For your References section:

    Human GBP1 is a microbe-specific gatekeeper of macrophage apoptosis and pyroptosis. Fisch D, Bando H, Clough B, Hornung V, Yamamoto M, Shenoy AR, Frickel EM. EMBO J. 2019 Jul 1;38(13):e100926. doi: 10.15252/embj.2018100926. Epub 2019 Jun 3. 10.15252/embj.2018100926 PubMed 31268602