Addgene - SIRT1 Flag Plasmid Data

Plasmid 13812: SIRT1 Flag

Gene/insert name:
SIRT1
Insert size:
2000
Species:
H. sapiens (human)
Entrez Gene:
SIRT1 (RP11-57G10.3, SIR2L1)
Fusion protein or tag:
flag
Terminal:
C terminal on backbone
Mutation:
N-terminus truncated
Vector backbone:
pcDNA3.1 +
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5500
Cloning site 5':
NotI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
BGH Reverse
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
Roy Frye
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Verdin
Terms and Licenses:
MTA

Comments:

cloned into a version of pcDNA3.1 that has been modified to include a C-terminal Flag tag (see author's map for details).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The human Sir2 ortholog, SIRT2, is an NAD+-dependent tubulin deacetylase. North et al (Mol Cell. 2003 Feb . 11(2):437-44. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13812" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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