pCiB-yin
(Plasmid #13845)

• Depositing Lab: Ting Wu
• Publication: Bateman et al Genetics. 2006 Jun . 173(2):769-77.

Backbone

• Vector backbone: pCar4
• Backbone size w/o insert (bp): 2500
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: yellow
• Alt name: y
• Species: D. melanogaster (fly)
• Insert Size (bp): 7000
• Entrez Gene: y (a.k.a. Dmel_CG3757, CG3757, Dmel\CG3757, EG:125H10.2, T6, Y)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: attB (not destroyed)
• 3′ cloning site: attB (not destroyed)
• 5′ sequencing primer: Pry1

Resource Information

• A portion of this plasmid was derived from a plasmid made by: attB from Michele Calos, Stanford

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pCiB-yin is a donor vector that contains an intronless yellow gene flanked by inverted attB sites. The cassette was constructed in the parent vector pCar4. In terms of use, there are no convenient restriction sites available to use pCiB-yin as a starting point for cloning (piB-GFP is more useful for that), but pCiB-yin provides a useful positive control for injections since insertion of the cassette produces a dominant pigmentation phenotype in a yellow background.