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RCAS(A) cScx HA (CT#744)
(Plasmid #13889)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    RCASBP(A)
  • Backbone size w/o insert (bp) 11600
  • Vector type
    Retroviral ; avian expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Scleraxis
  • Alt name
    Scx
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    600
  • Entrez Gene
    SCX
  • Tag / Fusion Protein
    • HA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer RCAS-F
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Andrew Lassar, Harvard
  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RCAS(A) cScx HA (CT#744) was a gift from Cliff Tabin (Addgene plasmid # 13889)
  • For your References section:

    A somitic compartment of tendon progenitors. Brent AE, Schweitzer R, Tabin CJ. Cell. 2003 Apr 18. 113(2):235-48. 10.1016/S0092-8674(03)00268-X PubMed 12705871