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pBS cPitx2 (CT#73)
(Plasmid #13927)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13927 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBS SK
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pitx2
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    1700
  • Entrez Gene
    PITX2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

full length original library isolate from Mackem dT, stage18-24 limb bud library. Used as an anti-sense riboprobe template and for subcloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS cPitx2 (CT#73) was a gift from Cliff Tabin (Addgene plasmid # 13927 ; http://n2t.net/addgene:13927 ; RRID:Addgene_13927)
  • For your References section:

    The transcription factor Pitx2 mediates situs-specific morphogenesis in response to left-right asymmetric signals. Logan M, Pagan-Westphal SM, Smith DM, Paganessi L, Tabin CJ. Cell. 1998 Aug 7. 94(3):307-17. 10.1016/S0092-8674(00)81474-9 PubMed 9708733