Addgene - RCAS(A) Chfz-7 WT (CT#532) Plasmid Data

Plasmid 13929: RCAS(A) Chfz-7 WT (CT#532)

Gene/insert name:
Fz 7
Alt name:
Frizzled 7
Species:
G. gallus (chicken)
Entrez Gene:
FZD7 (Fz-7, cFz-7)
Vector backbone:
RCASBP(A)
(Search Vector Database)
Vector type:
Retroviral ; avian expression
Backbone size w/o insert (bp):
11600
Cloning site 5':
ClaI
Site destroyed during cloning:
No
Cloning site 3':
ClaI
Site destroyed during cloning:
No
5' sequencing primer:
RCAS-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Principal Investigator:
Cliff Tabin
Terms and Licenses:
MTA

Comments:

Frizzled 7 wild type ORF: 5’ BsaI-primer, 3’ EcoRI-primer,
shuttle vector: Slax13.

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

Article: Dual roles of Wnt signaling during chondrogenesis in the chicken limb. Hartmann et al (Development. 2000 Jul . 127(14):3141-59. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13929" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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