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pGEX-4T3-p85beta-SH3
(Plasmid #1394)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1394 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pGEX-4T3
  • Backbone size w/o insert (bp) 4950
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PI3K regulatory subunit p85beta-SH3 domain
  • Alt name
    Pik3r2-SH3 domain (aa 1-80)
  • Alt name
    p85 alpha
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    240
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX-5
  • 3′ sequencing primer pGEX-3
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Claudine Yballe

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T3-p85beta-SH3 was a gift from Lewis Cantley (Addgene plasmid # 1394)

  • For your References section:

    Phosphoinositide 3-kinase binds constitutively to alpha/beta-tubulin and binds to gamma-tubulin in response to insulin. Kapeller R, Toker A, Cantley LC, Carpenter CL. J Biol Chem 1995 Oct 27;270(43):25985-91. 10.1074/jbc.270.43.25985 PubMed 7592789
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