Addgene - pGEM cAgg (CT#689) Plasmid Data

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Plasmid 13945: pGEM cAgg (CT#689)

Gene/insert name:
Aggrecan
Insert size:
687
Species:
G. gallus (chicken)
Entrez Gene:
ACAN (AGC1)
Mutation:
Nucleotides 113-800
Vector backbone:
pGEM-T Easy
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Bacterial Expression
Backbone size w/o insert:
3015
Cloning site 5':
NcoI
Site destroyed during cloning:
No
Cloning site 3':
SpeI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
SP6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Cliff Tabin
Terms and Licenses:
MTA

Comments:

687 bp partial clone of chick aggrecan, generated by RT-PCR corresponding to nucleotides 113-800 of Genbank sequence #L21913.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Wnt-14 plays a pivotal role in inducing synovial joint formation in the developing appendicular skeleton. Hartmann et al (Cell. 2001 Feb 9. 104(3):341-51. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13945" in your Materials and Methods section.