pGEM cCD44rel (CT#690)
(Plasmid #13946)

• Depositing Lab: Cliff Tabin
• Publication: Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

Backbone

• Vector backbone: pGEM-T Easy
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3015
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CD44 related
• Species: G. gallus (chicken)
• Insert Size (bp): 470
• Mutation: Nucleotides 814-1284
• Entrez Gene: CD44

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: NcoI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

470 bp partial cDNA clone of chick CD44rel amplified by RT-PCR corresponding to nucleotides 814-1284 of Genbank sequence #AF153205.

NOTE: Has a R323Q mutation compared GenBank. This mutation was not intentional, but it is not known to effect function.