Addgene - pBS cGli3 (CT#177) Plasmid Data

Plasmid 13954: pBS cGli3 (CT#177)

Gene/insert name:
Gli3
Alt name:
pcGli3b-19a
Insert size:
4500
Species:
G. gallus (chicken)
Entrez Gene:
GLI3 ()
Vector backbone:
pBS SK
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
3000
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
T3
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Cliff Tabin
Terms and Licenses:
MTA

Comments:

Starts at ~aa 430 of hGli3/mGli3.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Similar expression and regulation of Gli2 and Gli3 in the chick limb bud. Schweitzer et al (Mech Dev. 2000 Nov . 98(1-2):171-4. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13954" in your Materials and Methods section.