pLVX-Tight-Puro-KrasG12D2B
(Plasmid #139786)

• Purpose: Lentiviral tet-inducible expression of KrasG12D (mouse)
• Depositing Lab: Joan Massague
• Publication: Su et al Nature. 2020 Jan;577(7791):566-571. doi: 10.1038/s41586-019-1897-5. Epub 2020 Jan 8.

Backbone

• Vector backbone: pLVX-Tight-Puro
• Backbone size w/o insert (bp): 7791
• Total vector size (bp): 8358
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: KrasG12D
• Species: M. musculus (mouse)
• Insert Size (bp): 567
• Mutation: G12D
• Entrez Gene: Kras (a.k.a. K-Ras, K-Ras 2, K-ras, Ki-ras, Kras-2, Kras2, c-K-ras, c-Ki-ras, p21B, ras)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry