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Golgi-targeted optoPLD
(Plasmid #140058)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 140058 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCDNA5/FRT/TO
  • Backbone manufacturer
    Thermo Fisher Scientific
  • Backbone size w/o insert (bp) 5137
  • Total vector size (bp) 9587
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Phospholipase D
  • Species
    Streptomyces sp. PMF
  • Insert Size (bp)
    1518
  • GenBank ID
    UniProtKB P84147
  • Tags / Fusion Proteins
    • CRY2 (N terminal on insert)
    • mCherry (N terminal on insert)
    • P2A (C terminal on insert)
    • CIBN (C terminal on insert)
    • Sialyltransferase(TM) (C terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Golgi-targeted optoPLD was a gift from Jeremy Baskin (Addgene plasmid # 140058 ; http://n2t.net/addgene:140058 ; RRID:Addgene_140058)
  • For your References section:

    Spatiotemporal control of phosphatidic acid signaling with optogenetic, engineered phospholipase Ds. Tei R, Baskin JM. J Cell Biol. 2020 Mar 2;219(3). pii: 133675. doi: 10.1083/jcb.201907013. 10.1083/jcb.201907013 PubMed 31999306