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PurposeFor plant prime editing in rice plants or monocotyledons protoplasts
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 140447 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepHUE411
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Backbone manufacturerQi-Jun Chen (Addgene plasmid # 62203)
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Vector typePlant Expression, CRISPR
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namenCas9(H840A)-M-MLV
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Alt nameNLS-nCas9(H840A)-NLS-32aa linker-M-MLV-NLS
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SpeciesM. musculus (mouse); Streptococcus pyogene
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MutationH840A for Cas9; D200N, T306K, W313F, T330P and L603W for M-MLV
- Promoter maize Ubiquitin-1, OsU3
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pH-nCas9-PPE was a gift from Caixia Gao (Addgene plasmid # 140447 ; http://n2t.net/addgene:140447 ; RRID:Addgene_140447) -
For your References section:
Prime genome editing in rice and wheat. Lin Q, Zong Y, Xue C, Wang S, Jin S, Zhu Z, Wang Y, Anzalone AV, Raguram A, Doman JL, Liu DR, Gao C. Nat Biotechnol. 2020 May;38(5):582-585. doi: 10.1038/s41587-020-0455-x. Epub 2020 Mar 16. 10.1038/s41587-020-0455-x PubMed 32393904
