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pEIIIB
(Plasmid #14060)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14060 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEM-2
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2869
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rat Fibronectin EIIIB
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    255
  • Mutation
    EcoRI linkers were attached to HindIII - HinfI fragment.
  • GenBank ID
    L20801
  • Entrez Gene
    Fn1 (a.k.a. FIBNEC, fn-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEIIIB was a gift from Richard Hynes (Addgene plasmid # 14060)
  • For your References section:

    Cell-type-specific fibronectin subunits generated by alternative splicing. Paul JI, Schwarzbauer JE, Tamkun JW, Hynes RO. J Biol Chem. 1986 Sep 15. 261(26):12258-65. PubMed 3528152