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7iBi89 (rat fibronectin)
(Plasmid #14065)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14065 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBAGH.Sv
  • Backbone size w/o insert (bp) 7700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rat Fibronectin 7iBi89
  • Alt name
    Fibronectin 1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    3115
  • Mutation
    Insert was cut out of pGEM3 with SalI and SacI and blunt end cloned into BamHI sites.
  • GenBank ID
    L20801
  • Entrez Gene
    Fn1 (a.k.a. FIBNEC, fn-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    7iBi89 (rat fibronectin) was a gift from Richard Hynes (Addgene plasmid # 14065 ; http://n2t.net/addgene:14065 ; RRID:Addgene_14065)
  • For your References section:

    Elements regulating an alternatively spliced exon of the rat fibronectin gene. Huh GS, Hynes RO. Mol Cell Biol. 1993 Sep . 13(9):5301-14. PubMed 8355683