|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14067||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2869
Vector typeMammalian Expression
Growth in Bacteria
Gene/Insert nameChicken Beta 1 Integrin
SpeciesG. gallus (chicken)
Insert Size (bp)661
MutationPstI-BglI fragment was blunt end cloned and EcoRI linkers added and inserted in the EcoRI site.
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCINTBeta1 was a gift from Richard Hynes (Addgene plasmid # 14067)
For your References section:Structure of integrin, a glycoprotein involved in the transmembrane linkage between fibronectin and actin. Tamkun JW, DeSimone DW, Fonda D, Patel RS, Buck C, Horwitz AF, Hynes RO. Cell. 1986 Jul 18. 46(2):271-82. 10.1016/0092-8674(86)90744-0 PubMed 3487386
Map uploaded by the depositor.