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FUG-T2A-W274L
(Plasmid #140742)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 140742 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    FUG-PtenW274L
  • Backbone size w/o insert (bp) 9248
  • Total vector size (bp) 11246
  • Modifications to backbone
    The following oligos were annealed and cloned into the BsrG1 site of GFP to generate a T2A linker between the GFP-Pten. GTACAAGGAGGGCAGAGGAAGTCTTCTAACATGCGGTGACGTGGAGGAGAATCCCGGCCCTaA GTACTtAGGGCCGGGATTCTCCTCCACGTCACCGCATGTTAGAAGACTTCCTCTGCCCTCCTT
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GFP-T2A-PTENW274L
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1998
  • Mutation
    W274L
  • Entrez Gene
    PTEN (a.k.a. 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, TEP1)
  • Promoter hUbiC
  • Tag / Fusion Protein
    • none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrG1 (not destroyed)
  • 3′ cloning site BsrG1 (unknown if destroyed)
  • 5′ sequencing primer GFP Down
  • 3′ sequencing primer WRE up
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Pten insert from Alonzo Ross

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FUG-T2A-W274L was a gift from Bryan Luikart (Addgene plasmid # 140742 ; http://n2t.net/addgene:140742 ; RRID:Addgene_140742)
  • For your References section:

    Nuclear Excluded Autism-Associated Phosphatase and Tensin Homolog Mutations Dysregulate Neuronal Growth. Fricano-Kugler CJ, Getz SA, Williams MR, Zurawel AA, DeSpenza T Jr, Frazel PW, Li M, O'Malley AJ, Moen EL, Luikart BW. Biol Psychiatry. 2018 Aug 15;84(4):265-277. doi: 10.1016/j.biopsych.2017.11.025. Epub 2017 Dec 2. 10.1016/j.biopsych.2017.11.025 PubMed 29373119