Addgene - pBabe Nf2 delta BB Plasmid Data

Plasmid 14117: pBabe Nf2 delta BB

Gene/insert name:
Nf2 delta BB
Alt name:
Nf2
Species:
M. musculus (mouse)
Entrez Gene:
Nf2 (RP23-280J3.6, merlin)
Mutation:
delta BB: Blue Box residues deleted
Vector backbone:
pBabe-puro
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5169
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
pBABE 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
Tyler Jacks
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Cellular transformation by a FERM domain mutant of the Nf2 tumor suppressor gene. Johnson et al (Oncogene. 2002 Sep 5. 21(39):5990-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14117" in your Materials and Methods section.