pAG415GAL-ccdB-ECFP
(Plasmid #14169)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pRS415
  • Vector type
    Yeast Expression ; Gateway Destination
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    DB3.1, ccdB Survival
  • Copy number
    Unknown

Sequence Information

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • ECFP (C terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

Type of plasmid: CEN.

This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com

Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAG415GAL-ccdB-ECFP was a gift from Susan Lindquist (Addgene plasmid # 14169)