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pBabe puro IRES-EGFP Notes

SnaBI site does not exist

A digest of pBabe-puro-IRES-EGFP revealed that EcoRI linearizes this plasmid, but SnaBI does not.

The lanes are 1.Uncut DNA, 2.SnaBI digest, 3.EcoRI digest, 4.100bp ladder, 5.EcoRI + SnaBI double digest.

- Submitted by Christopher Valley, University of Minnesota

Addgene Diagnostic Digest for 14430

This gel depicts the NEB 1kb ladder and a digestion of the plasmid 14430: pBabe puro IRES-EGFP with the following enzymes: EcoRI-HF The expected pattern of bands was observed.