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pcDNA3.1-SARS-Spike
(Plasmid #145031)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 145031 Standard format: Plasmid sent in bacteria as agar stab 1 $75

Currently unavailable outside the U.S.

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 9300
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV Spike
  • Species
    Synthetic
  • Insert Size (bp)
    3840
  • Promoter CMV
  • Tags / Fusion Proteins
    • C9 (C terminal on insert)
    • CD5 signal peptide (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7-F
  • 3′ sequencing primer BGH-R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Please note that immediate availability of this material is restricted to U.S. customers only. For international customers, an export license is required. If you are interested in obtaining this plasmid, please contact [email protected]

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-SARS-Spike was a gift from Fang Li (Addgene plasmid # 145031 ; http://n2t.net/addgene:145031 ; RRID:Addgene_145031)
  • For your References section:

    Structural basis of receptor recognition by SARS-CoV-2. Shang J, Ye G, Shi K, Wan Y, Luo C, Aihara H, Geng Q, Auerbach A, Li F. Nature. 2020 Mar 30. pii: 10.1038/s41586-020-2179-y. doi: 10.1038/s41586-020-2179-y. 10.1038/s41586-020-2179-y PubMed 32225175