Addgene - pLV.ATRi Plasmid Data

Plasmid 14543: pLV.ATRi

Gene/insert name:
ATR shRNA
Alt name:
ATR
Insert size:
60
Species:
H. sapiens (human)
Entrez Gene:
ATR (FCTCS, FRP1, MEC1, SCKL, SCKL1)
Vector backbone:
pRDI292
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
SalI
Site destroyed during cloning:
No
5' sequencing primer:
H1 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
Didier Trono
Terms and Licenses:
MTA

Comments:

Sense oligo sequence used for cloning: ATR, 5'-GATCCCCGGC GTCGTCTCAG CTCGTCTTCA AGAGAGACGA GCTGAGACGA CGCCTTTTTG GAAA-3'.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: DNA damage sensors ATM, ATR, DNA-PKcs, and PARP-1 are dispensable for human immunodeficiency virus type 1 integration. Ariumi et al (J Virol. 2005 Mar . 79(5):2973-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14543" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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