Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pMSCV neo WT Akt1
(Plasmid #14549)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14549 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMSCV neo
  • Backbone manufacturer
    BD Biosciences
  • Backbone size w/o insert (bp) 6500
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Akt1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Akt1 (a.k.a. Akt, LTR-akt, PKB, PKB/Akt, PKBalpha, Rac)
  • Tag / Fusion Protein
    • HA tag

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Philip Tsichlis
  • Terms and Licenses

Depositor Comments

wild-type Akt1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV neo WT Akt1 was a gift from Joan Brugge (Addgene plasmid # 14549 ; http://n2t.net/addgene:14549 ; RRID:Addgene_14549)
  • For your References section:

    Distinct roles of Akt1 and Akt2 in regulating cell migration and epithelial-mesenchymal transition. Irie HY, Pearline RV, Grueneberg D, Hsia M, Ravichandran P, Kothari N, Natesan S, Brugge JS. J Cell Biol. 2005 Dec 19. 171(6):1023-34. 10.1083/jcb.200505087 PubMed 16365168