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TA p63 gamma rescue / pBabe puro
(Plasmid #14575)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14575 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pBABE-puro
  • Backbone manufacturer
    Available at Addgene (#1764)
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TA p63 gamma rescue
  • Alt name
    p63 gamma
  • Alt name
    p63g
  • Alt name
    p63
  • Species
    H. sapiens (human)
  • Mutation
    Transactivating (TA) isoform contains an amino-terminal exon that encodes a p53-like transactivation domain. shRNA rescue mutant contains three or four silent nucleotide changes to resist silencing by the shRNA described in the article.
  • Entrez Gene
    TP63 (a.k.a. AIS, B(p51A), B(p51B), EEC3, KET, LMS, NBP, OFC8, RHS, SHFM4, TP53CP, TP53L, TP73L, p40, p51, p53CP, p63, p73H, p73L)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI/SalI (destroyed during cloning)
  • 5′ sequencing primer pBabe 5'
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TA p63 gamma rescue / pBabe puro was a gift from Joan Brugge (Addgene plasmid # 14575)

  • For your References section:

    p63 regulates an adhesion programme and cell survival in epithelial cells. Carroll DK, Carroll JS, Leong CO, Cheng F, Brown M, Mills AA, Brugge JS, Ellisen LW. Nat Cell Biol. 2006 Jun . 8(6):551-61. 10.1038/ncb1420 PubMed 16715076
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