|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||145799||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
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Backbone manufacturermodified from pASK-IBA37plus (IBA GmbH)
Modifications to backboneRemoval of 6xHis tag, multiple cloning site, and Rop gene from pASK-IBA37plus
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameLarge-fragment Bst DNAP
Insert Size (bp)1827
- Promoter tet PA
/ Fusion Protein
- 6xHis (N terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer TACAGACCCTAATTTCACATCATATGAC
- 3′ sequencing primer CCGACGAACTAAAACGCTTGAGGAC (Common Sequencing Primers)
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAtetO_6xHis-Bst-LF was a gift from Andrew Ellington (Addgene plasmid # 145799 ; http://n2t.net/addgene:145799 ; RRID:Addgene_145799)
For your References section:High-surety isothermal amplification and detection of SARS-CoV-2, including with crude enzymes. Bhadra S, Riedel TE, Lakhotia S, Tran ND, Ellington AD. bioRxiv 2020.04.13.039941 10.1101/2020.04.13.039941