Addgene - pcDNA3 Tat HA (P#29) Plasmid Data

Plasmid 14654: pcDNA3 Tat HA (P#29)

Gene/insert name:
Tat
Insert size:
1080
Species:
HIV
Entrez Gene:
tat (HIV1gp5)
Fusion protein or tag:
HA
Terminal:
C terminal on insert
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5500
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Matija Peterlin
Terms and Licenses:
MTA

Comments:

Wild-type Tat gene, nucleotides 1-360.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The HIV transactivator TAT binds to the CDK-activating kinase and activates the phosphorylation of the carboxy-terminal domain of RNA polymerase II. Cujec et al (Genes Dev. 1997 Oct 15. 11(20):2645-57. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14654" in your Materials and Methods section.