pcDNA3 Tat HA (P#29)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14654||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)1080
Entrez Genetat (a.k.a. HIV1gp5)
/ Fusion Protein
- HA (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Wild-type Tat gene, nucleotides 1-360.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3 Tat HA (P#29) was a gift from Matija Peterlin (Addgene plasmid # 14654)
For your References section:The HIV transactivator TAT binds to the CDK-activating kinase and activates the phosphorylation of the carboxy-terminal domain of RNA polymerase II. Cujec TP, Okamoto H, Fujinaga K, Meyer J, Chamberlin H, Morgan DO, Peterlin BM. Genes Dev. 1997 Oct 15. 11(20):2645-57. 10.1101/gad.11.20.2645 PubMed 9334327