Addgene - pcDNA3 barr1 flag Plasmid Data

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Plasmid 14687: pcDNA3 barr1 flag

Gene/insert name:
beta arrestin 1
Alt name:
arrestin 2
Alt name:
arrb1
Insert size:
1300
Species:
R. norvegicus (rat)
GenBank ID:
NM_012910
Entrez Gene:
Arrb1 (BARRES)
Fusion protein or tag:
flag
Terminal:
C terminal on insert
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert:
5400
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
SP6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Supplemental document:
pcDNA3 Barr1 flag seq (application/pdf)
Principal Investigator:
Robert Lefkowitz
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Beta-arrestin-dependent formation of beta2 adrenergic receptor-Src protein kinase complexes. Luttrell et al (Science. 1999 Jan 29. 283(5402):655-61. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14687" in your Materials and Methods section.