Addgene - pRK5 bovine GRK2 Plasmid Data

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Plasmid 14691: pRK5 bovine GRK2

Gene/insert name:
GRK2
Alt name:
beta adrenergic receptor kinase 1
Alt name:
BARK1
Alt name:
ADRBK1
Insert size:
2300
Species:
B. taurus (bovine)
Entrez Gene:
ADRBK1 (BOS_24950, GRK2)
Vector backbone:
pRK5
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert:
4700
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
Sp6 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Robert Lefkowitz
Terms and Licenses:
MTA

Comments:

Using polymerase chain reaction, a 5'

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Feedback inhibition of G protein-coupled receptor kinase 2 (GRK2) activity by extracellular signal-regulated kinases. Pitcher et al (J Biol Chem. 1999 Dec 3. 274(49):34531-4. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14691" in your Materials and Methods section.