Addgene - pcDNA3 Flag beta-1-adrenergic-receptor Plasmid Data

Plasmid 14698: pcDNA3 Flag beta-1-adrenergic-receptor

Gene/insert name:
B1AR
Alt name:
beta-1 adrenergic receptor
Insert size:
1600
Species:
H. sapiens (human)
Entrez Gene:
ADRB1 (ADRB1R, B1AR, BETA1AR, RHR)
Fusion protein or tag:
Flag
Terminal:
N terminal on insert
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5400
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
SP6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Supplemental document:
pcDNA3 flag B1AR seq (application/pdf)
Principal Investigator:
Robert Lefkowitz
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Identification of the endophilins (SH3p4/p8/p13) as novel binding partners for the beta1-adrenergic receptor. Tang et al (Proc Natl Acad Sci U S A. 1999 Oct 26. 96(22):12559-64. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14698" in your Materials and Methods section.