Addgene - pSicoR human Drosha3 Plasmid Data

Plasmid 14768: pSicoR human Drosha3

Gene/insert name:
Drosha shRNA
Alt name:
Drosha
Alt name:
Rnasen
Species:
H. sapiens (human)
Entrez Gene:
DROSHA (ETOHI2, HSA242976, RANSE3L, RN3, RNASE3L, RNASEN)
Vector backbone:
pSicoR PGK puro
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi, Cre/Lox
Backbone size w/o insert (bp):
7700
5' sequencing primer:
mU6-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
Tyler Jacks
Terms and Licenses:
MTA

Comments:

shRNA sequence: TGAATTCGGC AGCCCAAATA TTCAAGAGAT ATTTGGGCTG CCGAATTCTT TTTTC

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Impaired microRNA processing enhances cellular transformation and tumorigenesis. Kumar et al (Nat Genet. 2007 Apr 1. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14768" in your Materials and Methods section.