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pSicoR human DGCR8-1
(Plasmid #14769)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14769 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSicoR PGK puro
  • Backbone size w/o insert (bp) 7700
  • Vector type
    Mammalian Expression, Lentiviral, RNAi, Cre/Lox
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DGCR8 shRNA
  • Alt name
    DGCR8
  • Species
    H. sapiens (human)
  • Entrez Gene
    DGCR8 (a.k.a. C22orf12, DGCRK6, Gy1, pasha)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

shRNA sequence: TGGGGTGAGA GTGCTGATAA TTCAAGAGAT TATCAGCACT CTCACCCCTT TTTTC

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSicoR human DGCR8-1 was a gift from Tyler Jacks (Addgene plasmid # 14769 ; http://n2t.net/addgene:14769 ; RRID:Addgene_14769)
  • For your References section:

    Impaired microRNA processing enhances cellular transformation and tumorigenesis. Kumar MS, Lu J, Mercer KL, Golub TR, Jacks T. Nat Genet. 2007 Apr 1. ():. 10.1038/ng2003 PubMed 17401365