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pSuper mouse Dicer3
(Plasmid #14778)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pSUPER.retro.puro
  • Backbone manufacturer
    OligoEngine
  • Backbone size w/o insert (bp) 6350
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Dicer shRNA
  • Alt name
    Dicer
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Dicer1 (a.k.a. 1110006F08Rik, D12Ertd7e, mKIAA0928)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

shRNA sequence: GATCCCCGCA TGGTGGTGTC GATATTTTCA AGAGAAATAT CGACACCACC ATGCTTTTTG GAAC

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSuper mouse Dicer3 was a gift from Tyler Jacks (Addgene plasmid # 14778)
  • For your References section:

    Impaired microRNA processing enhances cellular transformation and tumorigenesis. Kumar MS, Lu J, Mercer KL, Golub TR, Jacks T. Nat Genet. 2007 Apr 1. ():. 10.1038/ng2003 PubMed 17401365