Addgene - pSuper mouse Dicer3 Plasmid Data

Plasmid 14778: pSuper mouse Dicer3

Gene/insert name:
Dicer shRNA
Alt name:
Dicer
Species:
M. musculus (mouse)
Entrez Gene:
Dicer1 (1110006F08Rik, D12Ertd7e, mKIAA0928)
Vector backbone:
pSUPER.retro.puro
(Search Vector Database)
Backbone manufacturer:
OligoEngine
Vector type:
Mammalian Expression, Retroviral, RNAi
Backbone size w/o insert (bp):
6350
5' sequencing primer:
pLXSN5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
Tyler Jacks
Terms and Licenses:
MTA

Comments:

shRNA sequence: GATCCCCGCA TGGTGGTGTC GATATTTTCA AGAGAAATAT CGACACCACC ATGCTTTTTG GAAC

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Impaired microRNA processing enhances cellular transformation and tumorigenesis. Kumar et al (Nat Genet. 2007 Apr 1. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14778" in your Materials and Methods section.