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Plasmid 14797: pCAG-CreERT2
  • Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)

  • Cre-ERT2 fusion protein

  • inducible Cre

  • 2004

  • Bacteriophage P1

  • pCAGEN
    (Search Vector Database)

  • Mammalian Expression, Cre/Lox

  • 4779

  • EcoRI

  • No

  • NotI

  • No

  • pCAG-F List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • CreERT2 was modified from pCreERT2 (Feil et al. BBRC, 237, 752-757 (1997) obtained from Dr. P. Chambon (Universite Louis Pasteur).

  • View sequences (3)
  • View map

  • Connie Cepko

  • UBMTA
    IGBMC Cre-ERT2

Comments: 

4-hydroxitamoxifen-responsible Cre.
Kozak consensus sequence was added before the start ATG.
The insert can be cut out with EcoRI and NotI.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Controlled expression of transgenes introduced by in vivo electroporation. Matsuda et al (Proc Natl Acad Sci U S A. 2007 Jan 5. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14797" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only