Gene/insert name: Cre-ERT2 fusion protein
Alt name: inducible Cre
Insert size: 2004
Species: Bacteriophage P1
Vector backbone:
pCAGEN
(Search Vector Database )
Vector type:
Mammalian Expression, Cre/Lox
Backbone size w/o insert (bp):
4779
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
pCAG-F
List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
CreERT2 was modified from pCreERT2 (Feil et al. BBRC, 237, 752-757 (1997) obtained from Dr. P. Chambon (Universite Louis Pasteur).
Sequence:
View sequences (3)
Map:
View map
Principal Investigator:
Connie Cepko
Terms and Licenses:
MTA
Addgene has sequenced a portion of this plasmid for verification.
Click here for the sequencing
result.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
14797" in your Materials and Methods section.
4-hydroxitamoxifen-responsible Cre.
Kozak consensus sequence was added before the start ATG.
The insert can be cut out with EcoRI and NotI.