Addgene - pCAG-CreERT2 Plasmid Data

Gene/insert name:
Cre-ERT2 fusion protein
Alt name:
inducible Cre
Insert size:
2004
Species:
Bacteriophage P1
Vector backbone:
pCAGEN
(Search Vector Database)
Vector type:
Mammalian Expression, Cre/Lox
Backbone size w/o insert:
4779
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
pCAG-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
CreERT2 was modified from pCreERT2 (Feil et al. BBRC, 237, 752-757 (1997) obtained from Dr. P. Chambon (Universite Louis Pasteur).
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Connie Cepko
Terms and Licenses:
MTA

Comments:

4-hydroxitamoxifen-responsible Cre.
Kozak consensus sequence was added before the start ATG.
The insert can be cut out with EcoRI and NotI.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Controlled expression of transgenes introduced by in vivo electroporation. Matsuda et al (Proc Natl Acad Sci U S A. 2007 Jan 5. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14797" in your Materials and Methods section.