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pLCAG EGFP
(Plasmid #14857)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14857 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    LV-pGFP
  • Backbone manufacturer
    Naldini
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP

Cloning Information

Resource Information

Depositor Comments

CAG promoter was cloned into the ClaI and BamHI sites of LV-pGFP, replacing the PGK promoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLCAG EGFP was a gift from Inder Verma (Addgene plasmid # 14857 ; http://n2t.net/addgene:14857 ; RRID:Addgene_14857)
  • For your References section:

    Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos. Pfeifer A, Ikawa M, Dayn Y, Verma IM. Proc Natl Acad Sci U S A. 2002 Feb 19. 99(4):2140-5. 10.1073/pnas.251682798 PubMed 11854510