MyrPalm-CKAR
(Plasmid #14862)

• Depositing Lab: Alexandra Newton
• Publication: Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: C kinase activity reporter
• Alt name: CFP-FHA2-peptide-YFP
• Tags / Fusion Proteins:
  • Myr/Palm (N terminal on insert)
  • CFP (N terminal on insert)
  • YFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The MyrPalm tag in this plasmid uses the Lyn kinase amino terminal sequence of MGCIKSK from UniProtKB reference sequence P07948.3. This sequence encodes for myristoylation and palmitoylation, shown previously to be sufficient to target a protein to the plasma membrane.