pRSET B-CKAR
(Plasmid #14868)

• Depositing Lab: Alexandra Newton
• Publication: Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Backbone

• Vector backbone: pRSET B
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: C kinase activity reporter
• Alt name: CFP-FHA2-peptide-YFP
• Tags / Fusion Proteins:
  • 6xHis/Xpress (N terminal on backbone)
  • CFP (N terminal on insert)
  • YFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: XhoI (destroyed during cloning)
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

CKAR was amplified to include a 5' BglII site and a 3' SalI site and cloned into pRSETB cut from BamHI to XhoI.

Addgene QC NGS analysis identified a N68S discrepancy relative to Genbank ID 1QU5_A.