|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14884||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerI. Verma, Salk
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameflap-Ub promoter-GFP-WRE
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
Articles Citing this Plasmid
FG12 was derived from FUGW. The extra nucleotides from the HindIII site downstream of the Ubiquitin-C promoter (UbiC) promoter to the NcoI site in front of the initiation codon of EGFP were deleted by a HindIII-NcoI adapter ligation. Further, XbaI, EcoRI, and XhoI sites at the 3' end of EGFP and WRE were eliminated, followed by a polylinker oligonucleotide ligation at the PacI site between the Flap element and the UbiC promoter, to generate a set of new restriction sites, XbaI-HpaI-XhoI-BstXI-PacI, that is optimal for accommodating the siRNA expression cassette. To construct the siRNA-expressing lentiviral vectors, the siRNA expression cassette can be subcloned into FG12 between the XbaI and XhoI sites.
Lentiviruses can be produced with third generation packaging system plasmids pMDLg/pRRE, pRSV-Rev, and pMD2.G.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FG12 was a gift from David Baltimore (Addgene plasmid # 14884 ; http://n2t.net/addgene:14884 ; RRID:Addgene_14884)
For your References section:Inhibiting HIV-1 infection in human T cells by lentiviral-mediated delivery of small interfering RNA against CCR5. Qin XF, An DS, Chen IS, Baltimore D. Proc Natl Acad Sci U S A. 2003 Jan 7. 100(1):183-8. 10.1073/pnas.232688199 PubMed 12518064