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FG12
(Plasmid #14884)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14884 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    HR'CS-G
  • Backbone manufacturer
    I. Verma, Salk
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    flap-Ub promoter-GFP-WRE

Cloning Information

Resource Information

Depositor Comments

FG12 was derived from FUGW. The extra nucleotides from the HindIII site downstream of the Ubiquitin-C promoter (UbiC) promoter to the NcoI site in front of the initiation codon of EGFP were deleted by a HindIII-NcoI adapter ligation. Further, XbaI, EcoRI, and XhoI sites at the 3' end of EGFP and WRE were eliminated, followed by a polylinker oligonucleotide ligation at the PacI site between the Flap element and the UbiC promoter, to generate a set of new restriction sites, XbaI-HpaI-XhoI-BstXI-PacI, that is optimal for accommodating the siRNA expression cassette. To construct the siRNA-expressing lentiviral vectors, the siRNA expression cassette can be subcloned into FG12 between the XbaI and XhoI sites.

Lentiviruses can be produced with third generation packaging system plasmids pMDLg/pRRE, pRSV-Rev, and pMD2.G.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FG12 was a gift from David Baltimore (Addgene plasmid # 14884)
  • For your References section:

    Inhibiting HIV-1 infection in human T cells by lentiviral-mediated delivery of small interfering RNA against CCR5. Qin XF, An DS, Chen IS, Baltimore D. Proc Natl Acad Sci U S A. 2003 Jan 7. 100(1):183-8. 10.1073/pnas.232688199 PubMed 12518064