Addgene - pCMV5 HA FoxO1 Plasmid Data

Plasmid 14936: pCMV5 HA FoxO1

Gene/insert name:
FoxO1
Species:
M. musculus (mouse)
Entrez Gene:
Foxo1 (AI876417, Afxh, FKHR, Fkhr1, Foxo1a)
Fusion protein or tag:
HA
Terminal:
N terminal on backbone
Vector backbone:
HA-pCMV5
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Cloning site 5':
XbaI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
CMV Forward List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Joan Massague
Terms and Licenses:
MTA

Comments:

The XbaI fragment from Myc-FoxO1 was inserted into a modified pCMV5 vector containing an HA epitope to generate HA-FoxO1.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Integration of Smad and forkhead pathways in the control of neuroepithelial and glioblastoma cell proliferation. Seoane et al (Cell. 2004 Apr 16. 117(2):211-23. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14936" in your Materials and Methods section.