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pRK5 Flag PRAS40
(Plasmid #14950)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14950 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Flag-pRK5
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRAS40
  • Alt name
    AKT1S1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    800
  • Entrez Gene
    AKT1S1 (a.k.a. Lobe, PRAS40)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The cDNA for PRAS40 was PCR'd from a clone obtained from Origene.
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5 Flag PRAS40 was a gift from David Sabatini (Addgene plasmid # 14950 ; http://n2t.net/addgene:14950 ; RRID:Addgene_14950)
  • For your References section:

    PRAS40 is an insulin-regulated inhibitor of the mTORC1 protein kinase. Sancak Y, Thoreen CC, Peterson TR, Lindquist RA, Kang SA, Spooner E, Carr SA, Sabatini DM. Mol Cell. 2007 Mar 23. 25(6):903-15. 10.1016/j.molcel.2007.03.003 PubMed 17386266