Addgene - CS2 Flag-Smad6 Plasmid Data

Plasmid 14961: CS2 Flag-Smad6

Gene/insert name:
Smad6
Insert size:
1500
Species:
H. sapiens (human)
Entrez Gene:
SMAD6 (AOVD2, HsT17432, MADH6, MADH7)
Fusion protein or tag:
Flag
Terminal:
N terminal on insert
Vector backbone:
pCS2
(Search Vector Database)
Vector type:
xenopus/mammalian/avian/zebrafish
Backbone size w/o insert (bp):
4100
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
SP6 List of Sequencing Primers
3' sequencing primer:
EBV rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Joan Massague
Terms and Licenses:
MTA

Comments:

See author's map for more information.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Smad6 inhibits BMP/Smad1 signaling by specifically competing with the Smad4 tumor suppressor. Hata et al (Genes Dev. 1998 Jan 15. 12(2):186-97. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14961" in your Materials and Methods section.