CS2 Flag-Smad3 (EPSM)
(Plasmid #14963)

• Depositing Lab: Joan Massague
• Publication: Kretzschmar et al Genes Dev. 1999 Apr 1. 13(7):804-16.

Backbone

• Vector backbone: pCS2
• Backbone size w/o insert (bp): 4100
• Vector type: xenopus/mammalian/avian/zebrafish

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Smad3 (EPSM)
• Alt name: Smad3
• Species: H. sapiens (human)
• Insert Size (bp): 1300
• Mutation: T178V, S203A, S207A, S212A
• Entrez Gene: SMAD3 (a.k.a. HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, hMAD-3, hSMAD3, mad3)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: EBV rev

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

3SP and 1TP sites in the linker are mutated to alanine or valine (T-DV). See author's map for more information. Please note that the mutations correspond to residues 179, 204, 208, and 213 of the native Smad3 protein.