Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pBV-Luc/Del-7
(Plasmid #14968)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14968 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBV-luciferase
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    c-myc promoter (-26/+334)
  • Alt name
    c-myc promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    360
  • Mutation
    -26/+334 (relative to the P2 transcription initiation site) of the c-myc promoter.
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer LucNrev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

-26/+334 (relative to the P2 transcription initiation site) of the c-myc promoter was amplified by PCR and subcloned into the KpnI/EcoRI site of pBV-Luc, a low basal activity luciferase reporter plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBV-Luc/Del-7 was a gift from Joan Massague (Addgene plasmid # 14968 ; http://n2t.net/addgene:14968 ; RRID:Addgene_14968)
  • For your References section:

    Defective repression of c-myc in breast cancer cells: A loss at the core of the transforming growth factor beta growth arrest program. Chen CR, Kang Y, Massague J. Proc Natl Acad Sci U S A. 2001 Jan 30. 98(3):992-9. 10.1073/pnas.98.3.992 PubMed 11158583