Addgene - pBV-Luc/Del-7 Plasmid Data

Plasmid 14968: pBV-Luc/Del-7

Gene/insert name:
c-myc promoter (-26/+334)
Alt name:
c-myc promoter
Insert size:
360
Species:
H. sapiens (human)
Entrez Gene:
MYC (MRTL, bHLHe39, c-Myc)
Mutation:
-26/+334 (relative to the P2 transcription initiation site) of the c-myc promoter.
Vector backbone:
pBV-luciferase
(Search Vector Database)
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
4900
Cloning site 5':
KpnI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
LucNrev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Joan Massague
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

-26/+334 (relative to the P2 transcription initiation site) of the c-myc promoter was amplified by PCR and subcloned into the KpnI/EcoRI site of pBV-Luc, a low basal activity luciferase reporter plasmid.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Defective repression of c-myc in breast cancer cells: A loss at the core of the transforming growth factor beta growth arrest program. Chen et al (Proc Natl Acad Sci U S A. 2001 Jan 30. 98(3):992-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14968" in your Materials and Methods section.