pBV-Luc/Del-6
(Plasmid #14969)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pBV-luciferase
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    c-myc promoter (-109/+334)
  • Alt name
    c-myc promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    442
  • Mutation
    -109/+334 (relative to the P2 transcription initiation site) of the c-myc promoter.
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site PvuII (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer LucNrev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

XhoI to PvuII of c-myc promoter was subcloned into pBV-Luc, a low basal activity luciferase reporter plasmid.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBV-Luc/Del-6 was a gift from Joan Massague (Addgene plasmid # 14969)
  • For your References section:

    Defective repression of c-myc in breast cancer cells: A loss at the core of the transforming growth factor beta growth arrest program. Chen CR, Kang Y, Massague J. Proc Natl Acad Sci U S A. 2001 Jan 30. 98(3):992-9. 10.1073/pnas.98.3.992 PubMed 11158583