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pCX-EGFP beta5 integrin receptor
(Plasmid #14996)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14996 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCX-EGFP
  • Backbone size w/o insert (bp) 6182
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    beta5 integrin receptor
  • Alt name
    b5 integrin receptor
  • Species
    H. sapiens (human)
  • Entrez Gene
    ITGB5
  • Tag / Fusion Protein
    • IRES EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Human beta5 cDNA provided by Dr. David Cheresh. pCX-EGFP provided by Dr. Hidesaburo Hanafusa.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full-length human beta5 expression vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCX-EGFP beta5 integrin receptor was a gift from Raymond Birge (Addgene plasmid # 14996 ; http://n2t.net/addgene:14996 ; RRID:Addgene_14996)
  • For your References section:

    alphavbeta5 integrin recruits the CrkII-Dock180-rac1 complex for phagocytosis of apoptotic cells. Albert ML, Kim JI, Birge RB. Nat Cell Biol. 2000 Dec . 2(12):899-905. 10.1038/35046549 PubMed 11146654